产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human COL1A2 protein, and it does not cross react with human COL1A1, COL2A1, and COL3A1 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human COL1A2
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
collagen type I alpha 2 chain , EDSARTH2 , EDSCV , OI4
细胞定位
Secreted, extracellular space, extracellular matrix
功能与背景
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
数据库
研究领域
產品图片
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/templates/default/js/lightbox/images/loading.gif "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
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GTX637952 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637952 IHC-P Image
COL1A2 antibody [HL2048] detects secreted COL1A2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.COL1A2 stained by COL1A2 antibody [HL2048] (GTX637952) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637952 WB Image
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637952 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/small/GTX637952/GTX637952_44956_20230616_WB_D_23062019_595.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952](/upload/website/prouct_img/small/GTX637952/GTX637952_T-44886_20221230_IHC-P_multiple_M_23020621_347.webp "Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/small/GTX637952/GTX637952_44956_20230217_WB_Fraction_23022022_489.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/small/GTX637952/GTX637952_44956_20230331_WB_multiple_B_23041023_496.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/normal/GTX637952/GTX637952_44956_20230616_WB_D_23062019_595.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952](/upload/website/prouct_img/normal/GTX637952/GTX637952_T-44886_20221230_IHC-P_multiple_M_23020621_347.webp "Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/normal/GTX637952/GTX637952_44956_20230217_WB_Fraction_23022022_489.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
![Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952](/upload/website/prouct_img/normal/GTX637952/GTX637952_44956_20230331_WB_multiple_B_23041023_496.webp "Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952")
