产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Raji
预测种属反应
Mouse, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human COPB. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950017
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
coatomer protein complex subunit beta 1 , COPB
细胞定位
Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa
功能与背景
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113167 IHC-P Image
COPB antibody [N1N2], N-term detects COPB protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. COPB antibody [N1N2], N-term (GTX113167) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113167 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using COPB(GTX113167) antibody at 1:100 dilution.
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GTX113167 WB Image
Sample (30 ug of whole cell lysate) A: Raji
7.5% SDS PAGE
GTX113167 diluted at 1:1000
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GTX113167 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using COPB(GTX113167) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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