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COX4抗体 [GT854]

KO/KD Orthogonal
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

目录号 GTX628901

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT854

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 COX4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

20 kDa. ( Note )

阳性对照

mouse heart , rat muscle , 293T

预测种属反应

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888077

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

细胞定位

Mitochondrion inner membrane

功能与背景

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody detects COX4I1 protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
12% SDS-PAGE
COX4 antibody (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901

GTX628901 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX628901) diluted at 1:1000.

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.
A. 50 μg rat muscle lysate/extract
15% SDS-PAGE
COX4 antibody [GT854] (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Zhao J et al. Br J Cancer 2021; 124 (2) : 425-436 G3BP1 interacts with YWHAZ to regulate chemoresistance and predict adjuvant chemotherapy benefit in gastric cancer.
Peri? I et al. Neuroscience 2020; 451 : 111-125 Tianeptine Enhances Energy-related Processes in the Hippocampal Non-synaptic Mitochondria in a Rat Model of Depression.
实验应用 : WB
种属反应 : Rat
Dongil P et al. Aging (Albany NY) 2020; 12 (3) : 2275-2301 PAS kinase deficiency reduces aging effects in mice.
实验应用 : WB
种属反应 : Mouse
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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