产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human COX4.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1
细胞定位
Mitochondrion inner membrane
功能与背景
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
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GTX638316 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638316 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638316 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638316 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX638316 IHC-P Image
COX4 antibody [HL2264] detects COX4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.COX4 stained by COX4 antibody [HL2264] (GTX638316) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638316 ICC/IF Image
COX4 antibody [HL2264] detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody [HL2264] (GTX638316) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638316 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638316 WB Image
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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