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CREB (phospho Ser133)抗体 [HL2776]

Recombinant antibody Orthogonal
Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641
Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641
Anti-CREB (phospho Ser133) antibody [HL2776] used in IHC (Paraffin sections) (IHC-P). GTX639641

目录号 GTX639641

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2776

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CREB 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Ser133 of human CREB1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cAMP responsive element binding protein 1 , CREB , CREB-1

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

数据库

研究领域

產品图片

Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641

GTX639641 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641

GTX639641 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CREB (phospho Ser133) antibody [HL2776] used in IHC (Paraffin sections) (IHC-P). GTX639641

GTX639641 IHC-P Image

CREB (phospho Ser133) antibody [HL2776] detects CREB (phospho Ser133) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
CREB (phospho Ser133) stained by CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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