产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:5000-1:100000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , rat brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS
预测种属反应
Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human CRMP2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036670
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
dihydropyrimidinase like 2 , CRMP-2 , CRMP2 , DHPRP2 , DRP-2 , DRP2 , N2A3 , ULIP-2 , ULIP2
细胞定位
Cytoplasm
功能与背景
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
数据库
研究领域
產品图片
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GTX113420 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113420 IHC-P Image
CRMP2 antibody [N1N3] detects CRMP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113420 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113420 ICC/IF Image
CRMP2 antibody [N1N3] detects CRMP2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113420 IHC-P Image
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using CRMP2(GTX113420) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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