NCI-H520 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

CTLA4 antibody [HL2732] detects CTLA4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human spleen.
CTLA4 stained by CTLA4 antibody [HL2732] (GTX639560) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.