产品说明
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:50-1:100 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.
Negative Control
Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA (Please contact us for PBS only format)
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human CTLA4 protein
纯化方式
Protein A/G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cytotoxic T-lymphocyte associated protein 4 , ALPS5 , CD , CD152 , CELIAC3 , CTLA-4 , GRD4 , GSE , IDDM12
细胞定位
Cell membrane
功能与背景
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX04439 IHC-P Image
IHC-P analysis of human diffuse large B-cell lymphoma tissue section using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX™. Diffuse large B cell lymphoma containing a large number of CTLA4 positive lymphocytes.
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GTX04439 IHC-P Image
IHC-P analysis of human Hodgkin's lymphoma tissue section using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX. Hodgkins lymphoma containing numerous reactive CTLA4 positive lymphocytes.
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GTX04439 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX04439 CTLA4 antibody [MSVA-152R] HistoMAX™. Normal tonsil with CTLA4 staining of a subset of lymphocytes.
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文献引用
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产品评论
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