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CXCL8 / IL8抗体

Orthogonal Overexpression
Anti-CXCL8 / IL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

目录号 GTX115959

文献引用 6
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, FACS

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

11 kDa. ( Note )

阳性对照

IL8-transfected 293T , Human leukocyte

预测种属反应

Sheep, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human CXCL8 / IL8 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11167915

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

C-X-C motif chemokine ligand 8 , GCP-1 , GCP1 , IL8 , LECT , LUCT , LYNAP , MDNCF , MONAP , NAF , NAP-1 , NAP1

细胞定位

Secreted

功能与背景

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CXCL8 / IL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX115959

GTX115959 IHC-P Image

CXCL8 / IL8 antibody detects secreted CXCL8 / IL8 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric cancer.
CXCL8 / IL8 stained by CXCL8 / IL8 antibody (GTX115959) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

Human leukocyte (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8 / IL8 antibody (GTX115959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959

GTX115959 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26465338

文献引用

Application Reference

Chang MC et al. J Formos Med Assoc 2018; (Epub) IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38.
实验应用 : WB
种属反应 : Human
Lin SI et al. J Formos Med Assoc 2018; (Epub) IL-1β induced IL-8 and uPA expression/production of dental pulp cells: Role of TAK1 and MEK/ERK signaling.
实验应用 : WB
种属反应 : Human
Liu J et al. Cancer Res 2018; (Epub) Differential effects of estrogen receptor beta isoforms on glioblastoma progression.
实验应用 : IHC-P
种属反应 : Human
Tsai CH et al. Sci Rep 2017; 7 (1) : 15624 A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of * in prostate cancer.
实验应用 : IHC-P
种属反应 : Human
Chen WC et al. PLoS One 2015; 10 (7) : e0133264 HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway.
实验应用 : WB
种属反应 : Human
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产品评论

CXCL8 / IL8 antibody 目录号 GTX115959

Rating
( Average 4.5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-CXCL8 / IL8 antibody used in Flow cytometry (FACS). GTX115959
Date : Anonymous submitted on 20-Apr-2018
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Permeabilization /Antigen retrieval : 0.05% Tween-20 in PBS
Primary Antibody : 1:100, 25°C, 1Hr
Anti-CXCL8 / IL8 antibody used in Western Blot (WB). GTX115959
Date : Anonymous submitted on 28-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 50 μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Culture supernatant: 800ul culture medium TCA precipitation.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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