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CXCR2抗体 [HL2604]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056
Anti-CXCR2 antibody [HL2604] used in IHC (Paraffin sections) (IHC-P). GTX639056
Anti-CXCR2 antibody [HL2604] used in Functional Assay (Functional Assay). GTX639056
Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056

目录号 GTX639056

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2604

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, Functional Assay

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Functional Assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

41 kDa. ( Note )

产品说明

This antibody was raised against human CXCR2 Extracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Extracellular domain of human CXCR2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

C-X-C motif chemokine receptor 2 , CD182 , CDw128b , CMKAR2 , IL8R2 , IL8RA , IL8RB

细胞定位

Cell membrane; Multi-pass membrane protein

功能与背景

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

数据库

研究领域

產品图片

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Comparison Orthogonal

Unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and competitor's antibody (Clone 48311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Comparison Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056

GTX639056 ICC/IF Image

Comparison

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:200 and competitor's antibody (Highly Cited Competitor) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Overexpression

Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR2 antibody [HL2604] used in Western Blot (WB). GTX639056

GTX639056 WB Image

Orthogonal

Unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCR2 antibody [HL2604] used in IHC (Paraffin sections) (IHC-P). GTX639056

GTX639056 IHC-P Image

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR2 antibody [HL2604] used in Functional Assay (Functional Assay). GTX639056

GTX639056 Functional Assay Image

CXCR2 antibody [HL2604] (GTX639056) specifically detects CXCR2 protein on a VirDTM-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:1000 dilution.

Yellow: CXCR2
Blue: CXCR1, CXCR3, CXCR4, CXCR5, or CXCR6
White: Positive control

Anti-CXCR2 antibody [HL2604] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639056

GTX639056 ICC/IF Image

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in MeOH.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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