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CXCR4抗体 [HL2612]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

目录号 GTX639064

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2612

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Human, Mouse, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

40 kDa. ( Note )

产品说明

This antibody was raised against human CXCR4 Intracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR4. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

细胞定位

Cell membrane,Cell junction,Early endosome,Lysosome

功能与背景

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

KO/KD Orthogonal

Non-transfected (–) and transfected (+) Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639064

GTX639064 ICC/IF Image

CXCR4 antibody [HL2612] detects CXCR4 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Orthogonal

Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
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¥ 1700
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