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CXCR7抗体 [HL2189]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193
Anti-CXCR7 antibody [HL2189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193
Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

目录号 GTX638193

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2189

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

41 kDa. ( Note )

产品说明

This antibody was raised against human CXCR7 Intracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR7. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

atypical chemokine receptor 3 , CMKOR1 , CXC-R7 , CXCR-7 , CXCR7 , GPR159 , RDC-1 , RDC1

细胞定位

Cell membrane; Multi-pass membrane protein,Cytoplasm, perinuclear region,Early endosome

功能与背景

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

KO/KD

Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

Comparison

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:200 and competitor's antibody (MAB42273) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Orthogonal

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in IHC (Paraffin sections) (IHC-P). GTX638193

GTX638193 IHC-P Image

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CXCR7 antibody [HL2189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638193

GTX638193 ICC/IF Image

Overexpression

CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR7 antibody [HL2189] used in Western Blot (WB). GTX638193

GTX638193 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

P Meng et al. Ren Fail 2024; 46 (1) : 2300727 CXC chemokine receptor 7 ameliorates renal fibrosis by inhibiting β-catenin signaling and epithelial-to-mesenchymal transition in tubular epithelial cells
实验应用 : IHC-Fr
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产品包装 报价 (¥)
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¥ 1700
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