Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody (GTX103718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CYP27A1(GTX103718) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CYP27A1 antibody detects CYP27A1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
7.5% SDS-PAGE
CYP27A1 antibody (GTX103718) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CYP27A1 antibody (GTX103718) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min