产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse liver , rat liver , A549
预测种属反应
Rabbit, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.33 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11168982
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cytochrome P450 family 27 subfamily A member 1 , CP27 , CTX , CYP27
细胞定位
Mitochondrion membrane
功能与背景
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX103718 IHC-P Image
CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CYP27A1 antibody (GTX103718) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX103718 WB Image
Sample (50 μg of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX103718 WB Image
CYP27A1 antibody detects CYP27A1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
7.5% SDS-PAGE
CYP27A1 antibody (GTX103718) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX103718 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CYP27A1(GTX103718) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX103718 WB Image
Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX103718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX103718 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody (GTX103718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
文献引用
产品评论
目前没有使用 CYP27A1 antibody (GTX103718) 的产品评论被收录。快发表第一篇产品评论吧！
