产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Calbindin
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calbindin 1 , CALB , D-28K
细胞定位
Nucleus,Cytosol
功能与背景
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
数据库
研究领域
產品图片
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/templates/default/js/lightbox/images/loading.gif "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
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GTX637439 WB Image
Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637439 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637439 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX637439 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637439 IHC-Fr Image
Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637439 IHC-P Image
Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637439 IHC-P Image
Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637439 ICC/IF Image
Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_T-44802_20230106_WB_brain_23010922_799.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_T-44802_20230106_WB_multiple_B_23010922_951.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_44942_20230210_WB_23021401_751.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_44942_20230224_WB_M_R_23030219_743.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_44942_20230526_IHC-Fr_M_23053001_612.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_T-44802_20221209_IHC-P_M_22121123_329.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_T-44802_20221209_IHC-P_R_22121123_588.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439](/upload/website/prouct_img/small/GTX637439/GTX637439_T-44802_20221209_ICC_IF_R_22122018_114.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_T-44802_20230106_WB_brain_23010922_799.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_T-44802_20230106_WB_multiple_B_23010922_951.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_44942_20230210_WB_23021401_751.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_44942_20230224_WB_M_R_23030219_743.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_44942_20230526_IHC-Fr_M_23053001_612.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_T-44802_20221209_IHC-P_M_22121123_329.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_T-44802_20221209_IHC-P_R_22121123_588.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439")
![Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439](/upload/website/prouct_img/normal/GTX637439/GTX637439_T-44802_20221209_ICC_IF_R_22122018_114.webp "Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439")
