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Calbindin抗体 [GT386]

Anti-Calbindin antibody [GT386] used in Western Blot (WB). GTX634833
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

目录号 GTX634833

文献引用 1
3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT386

同种型

 IgG2a

实验应用

 WB, IHC-P, IHC (Free Floating)

种属反应

 Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
IHC-P Assay dependent
IHC (Free Floating) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

30 kDa. ( Note )

预测种属反应

Human, Rabbit, Bovine, Chicken, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Calbindin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888488

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

calbindin 1 , CALB , D-28K

功能与背景

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

数据库

研究领域

產品图片

Anti-Calbindin antibody [GT386] used in Western Blot (WB). GTX634833

GTX634833 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [GT386] (GTX634833) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
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产品评论

Calbindin antibody [GT386] 目录号 GTX634833

Rating
( Average 5 based on 3 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Free Floating)(IHC (Free Floating))
( Average 5 based on 2 users reviews)
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Date : Estela Maris Muñoz submitted on 08-Apr-2022
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat cerebellum tissue section
Blocking : 10% Normal Donkey Serum Buffer, 25°C, 1Hr
Fixation : 4% PFA in PBS, 4°C, > 12Hr
Permeabilization /Antigen retrieval : 10 mM citrate buffer (pH6), 0.5Hr
Primary Antibody : 1:500 diluted in PBS with 2% NDSB and 1% Triton X-100, 4°C, 16Hr
Figure Note : IHC and confocal microscopy for a calbindin-positive Purkinje cell present in the cerebellum of an adult male Wistar rat.
A1-A2: Immunolabeling for calbindin (Calb; red). Nuclei (Nu) were counterstained with DAPI (grey). Purkinje neurons (PkC) express calbindin in their somas and projections. These GABAergic cells exhibit an intricately elaborate dendritic arbor towards the cerebellar molecular layer (yellow arrow) and a single long axon (cyan arrow) that forms synapsis in the deep cerebellar nuclei.
A3: 3D reconstruction of the main projections of the Purkinje cell pointed in A and B. Image generated at 60X with 2.0 zoom, in an Olympus FV1000 confocal microscope.
Authors: Martin Avila and Estela Muñoz (IHEM-UNCuyo-CONICET, Mendoza, Argentina).
Anti-Calbindin antibody [GT386] used in IHC (Free Floating) (IHC (Free Floating)). GTX634833
Date : Anonymous submitted on 23-Jun-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse Brain cerebellum 250μm slice
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Figure Note : Calbindin (red) & VGLUT2 (green, GTX133142)
Anti-Calbindin antibody [GT386] used in IHC (Free Floating) (IHC (Free Floating)). GTX634833
Date : Wei-Hao Liao submitted on 04-Jun-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse Brain cerebellum 250μm slice
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
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SDS
PBS.pdf
Glycerol.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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