产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , rat brain
产品说明
Based on sequence homology, this antibody may cross react with CALB2 protein.
预测种属反应
Rabbit, Bovine, Chicken, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.12 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Calbindin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886366
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calbindin 1 , CALB , D-28K
功能与背景
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX130856 IHC-Fr Image
Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130856 IHC-Fr Image
Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130856 IHC-P Image
Calbindin antibody detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130856 IHC-P Image
Calbindin antibody detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130856 WB Image
IMR32 whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130856 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130856 IHC-P Image
Calbindin antibody detects Calbindin protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130856 IHC-P Image
Calbindin antibody detects Calbindin protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130856 IHC-P Image
Calbindin antibody detects Calbindin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130856 IHC-Fr Image
Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Calbindin protein stained by Calbindin antibody (GTX133218) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130856 ICC/IF Image
Calbindin antibody detects Calbindin protein at nucleus and cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX130856 WB Image
Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130856 ICC/IF Image
Calbindin antibody detects Calbindin protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX130856 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Calbindin antibody (GTX130856) diluted at 1:1000 and competitor's antibody (sc-7691) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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