产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human Calcium Sensing Receptor Extracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human Calcium Sensing Receptor. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calcium sensing receptor , CAR , EIG8 , FHH , FIH , GPRC2A , HHC , HHC1 , HYPOC1 , NSHPT , PCAR1 , hCasR
细胞定位
Cell membrane
功能与背景
The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
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GTX638563 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000 and competitor's antibody (Clone 5C10, ADD) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638563 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638563 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638563 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638563 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638563 IP Image
Immunoprecipitation of Calcium Sensing Receptor protein from TT whole cell extract using 5 μg of Calcium Sensing Receptor antibody [HL2357] (GTX638563). Western blot analysis was performed using Calcium Sensing Receptor antibody [HL2357] (GTX638563). EasyBlot HRP-conjugated anti FALSE.
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