GeneTex中国官方网站
  • China

Calnexin抗体 [GT1563]

KO/KD Overexpression IP/MS
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976
Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

目录号 GTX629976

文献引用 5

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT1563

同种型

 IgG2a

实验应用

 WB, ICC/IF, IP, Dot

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Calnexin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Dot Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

mouse liver , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2

产品说明

IP/MS validation was supported by references (PMID:30377428)

预测种属反应

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888183

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

calnexin , CNX , IP90 , P90

细胞定位

Endoplasmic reticulum membrane , Melanosome

功能与背景

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:500

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

GTX629976 ICC/IF Image

Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500.
Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

GTX629976 ICC/IF Image

Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

Azalea A Khan et al. Bioconjug Chem 2022; 33 (3) : 473-485 PET Imaging of Small Extracellular Vesicles via [89Zr]Zr(oxinate)4 Direct Radiolabeling.
实验应用 : Dot
种属反应 : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
实验应用 : WB
AA Khan et al. Anal Chem 2020; [89Zr]Zr(oxinate)4 Allows Direct Radiolabelling and PET Imaging of Small Extracellular Vesicles
Gao Z et al. Antiviral Res 2020; 182 : 104907 Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.
实验应用 : WB
种属反应 : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
实验应用 : IP
种属反应 : Human
我要回报文献

产品评论

目前没有使用 Calnexin antibody [GT1563] (GTX629976) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com