产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calnexin , CNX , IP90 , P90
细胞定位
Endoplasmic reticulum membrane,Endoplasmic reticulum
功能与背景
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
数据库
研究领域
產品图片
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GTX637077 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat mammary gland.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 IHC-P Image
Calnexin antibody [HL1598] detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637077 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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