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Calpain 1抗体 [N3C2], Internal

Orthogonal
Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

目录号 GTX102340

文献引用 8
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Squirrel
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Calpain 1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

82 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2

预测种属反应

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618731

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL

功能与背景

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340

GTX102340 IP Image

Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

GTX102340 WB Image

Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340

GTX102340 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.

Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

GTX102340 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35163312

文献引用

Application Reference

E Fletcher et al. Transl Res 2023; S1931-5244 (23) : 00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
实验应用 : WB
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.
实验应用 : WB
种属反应 : Human
Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
Lin JH et al. Phytother Res 2020;
实验应用 : WB
种属反应 : Human
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产品评论

Calpain 1 antibody [N3C2], Internal 目录号 GTX102340

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
我要评论产品
Application Reference
E Fletcher et al. Transl Res 2023; S1931-5244 (23):00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
实验应用 : WB
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.
实验应用 : WB
种属反应 : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
Lin JH et al. Phytother Res 2020;
实验应用 : WB
种属反应 : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
实验应用 : WB
种属反应 : Human
Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
实验应用 : WB
种属反应 : Human
Zhang Y et al. Cell Stress Chaperones 2016; (Epub) Regulation of gene expression by NFAT transcription factors in hibernating ground squirrels is dependent on the cellular environment.
实验应用 : WB
种属反应 : Squirrel
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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