产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Jurkat
预测种属反应
Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.55 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10729181
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL
细胞定位
Cytoplasm , Cell membrane
功能与背景
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX102350 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (GTX102350) at 1:300 dilution.
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GTX102350 WB Image
Sample (30 μg of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE GTX102350 diluted at 1:1000
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GTX102350 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1(GTX102350) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX102350 WB Image
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE GTX102350 diluted at 1:1000
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GTX102350 IHC-P Image
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1(GTX102350) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献引用
产品评论
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