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Carbonic Anhydrase IX抗体 [GT12]

Orthogonal
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Flow cytometry (FACS). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020
Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

目录号 GTX70020

文献引用 12

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT12

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

种属反应

 Human
产品包装
100 μl (¥4300)
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产品说明

摘要

Carbonic Anhydrase IX antibody (CA9 antibody) detects carbonic anhydrase 9, a ~50 kDa transmembrane glycoprotein. CA9 expression is enhanced by HIF1-alpha signaling in various biological processes, including cell proliferation and transformation. Found in many tissues, CA9 overexpression promotes tumor growth in various cancers.

产品应用

应用说明

For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10μg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.

预期分子量

50 kDa. ( Note )

产品说明

Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

偶联

Unconjugated

RRID

AB_11173476

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

carbonic anhydrase 9 , CAIX , MN

细胞定位

Nucleus , Cell membrane , Nucleolus

功能与背景

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

数据库

研究领域

產品图片

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Flow cytometry (FACS). GTX70020

GTX70020 FACS Image

Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70020

GTX70020 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

GTX70020 WB Image

Orthogonal

Sample (30 μg HeLa whole cell lysate)

A: 24 hr Untreated

B: 24 hr treatment with 100μM CoCl2

C: 24 hr treatment with 200μM CoCl2

D: 48 hr Untreated

E: 48 hr treatment with 100μM CoCl2

F: 48 hr treatment with 200μM CoCl2



Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.

Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

GTX70020 WB Image

Orthogonal

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

GTX70020 WB Image

Orthogonal

The data was published in the journal PLoS One in 2014. PMID: 25420025

Anti-Carbonic Anhydrase IX antibody [GT12] used in IHC (Paraffin sections) (IHC-P). GTX70020

GTX70020 IHC-P Image

The data was published in the journal PLoS One in 2015. PMID: 25738958

Anti-Carbonic Anhydrase IX antibody [GT12] used in Western Blot (WB). GTX70020

GTX70020 WB Image

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

文献引用

Application Reference

Tang X et al. Cell Prolif 2021; 54 (9) : e13112 The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture.
实验应用 : IHC-Fr
种属反应 : Human
Davis RM et al. ACS Nano 2018; 12 (10) : 9669-9679 Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
Thompson JM et al. Mol Cancer Ther 2018; (Epub) Targeting the mevalonate pathway suppresses VHL-deficient CC-RCC through a HIF-dependent mechanism.
实验应用 : WB
种属反应 : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1) : 70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
实验应用 : WB
种属反应 : Human
Jin X et al. Nat Med 2017; 23 (11) : 1352-1361 Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.
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Application Reference
Tang X et al. Cell Prolif 2021; 54 (9):e13112 The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture.
实验应用 : IHC-Fr
种属反应 : Human
Davis RM et al. ACS Nano 2018; 12 (10):9669-9679 Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
Thompson JM et al. Mol Cancer Ther 2018; (Epub) Targeting the mevalonate pathway suppresses VHL-deficient CC-RCC through a HIF-dependent mechanism.
实验应用 : WB
种属反应 : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1):70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
实验应用 : WB
种属反应 : Human
Jin X et al. Nat Med 2017; 23 (11):1352-1361 Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.
Ramchandani D et al. Lab Invest 2016; 96 (12):1234-1245 Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions.
实验应用 : WB
种属反应 : Human
Abd-Aziz N et al. Oncol Lett 2015; 10 (4):2192-2196 * attenuates hif-1- but not hif-2-mediated transcriptional activation.
实验应用 : WB
种属反应 : Human
Huang WJ et al. PLoS One 2015; 10 (3):e0119181 Expression of hypoxic marker carbonic anhydrase IX predicts poor prognosis in resectable hepatocellular carcinoma.
实验应用 : IHC
种属反应 : Human
Hou P et al. PLoS One 2014; 9 (11):e113865 Intermediary * Precursor Dimethyl-2-Ketoglutarate Stabilizes Hypoxia-Inducible Factor-1α by Inhibiting Prolyl-4-Hydroxylase PHD2.
实验应用 : WB
种属反应 : Human
Wiegering A et al. Neoplasia 2014; 16 (11):972-81 E7080 (lenvatinib), a multi-targeted tyrosine kinase inhibitor, demonstrates antitumor activities against colorectal cancer xenografts.
实验应用 : IHC-Fr
种属反应 : Human
Pritchett TL et al. Oncogene 2014; 0 Conditional inactivation of the mouse von Hippel-Lindau tumor suppressor gene results in wide-spread hyperplastic, inflammatory and fibrotic lesions in the kidney.
实验应用 : IHC-P
Hwang-Verslues WW et al. Proc Natl Acad Sci U S A 2013; 110 (30):12331-6 Loss of corepressor PER2 under hypoxia up-regulates OCT1-mediated EMT gene expression and enhances tumor malignancy.
实验应用 : IHC
种属反应 : Human
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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