GeneTex中国官方网站
  • China

Carbonic Anhydrase IX抗体 [HL1410]

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876
Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876

目录号 GTX636876

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL1410

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

产品说明

This antibody was raised against human Carbonic Anhydrase IX Extracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

carbonic anhydrase 9 , CAIX , MN

细胞定位

Nucleus,Cell membrane,Nucleolus

功能与背景

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

数据库

研究领域

產品图片

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876

GTX636876 IHC-P Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [HL1410] used in IHC (Paraffin sections) (IHC-P). GTX636876

GTX636876 IHC-P Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Carbonic Anhydrase IX antibody [HL1410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636876

GTX636876 ICC/IF Image

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Carbonic Anhydrase IX antibody [HL1410] used in Western Blot (WB). GTX636876

GTX636876 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

目前没有使用 Carbonic Anhydrase IX antibody [HL1410] (GTX636876) 的文献被收录。快来回报我们第一篇参考文献吧!

产品评论

目前没有使用 Carbonic Anhydrase IX antibody [HL1410] (GTX636876) 的产品评论被收录。快发表第一篇产品评论吧!
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com