产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human Cardiac Troponin I recombinant protein
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI
功能与背景
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX636963 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636963 IHC-P Image
Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636963 IHC-P Image
Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636963 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636963 IHC-P Image
Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat heart.Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636963 WB Image
Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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