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Cardiac Troponin I抗体 [N1C3-3]

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

目录号 GTX113028

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC

种属反应

Human, Mouse, Pig
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

阳性对照

Raji , mouse heart

预测种属反应

Rat, Bovine, Cat, Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10722789

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

功能与背景

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113028 diluted at 1:1000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX113028 diluted at 1:5000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

GTX113028 ICC/IF Image

Cardiac Troponin I antibody [N1C3-3] detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cardiac Troponin I protein stained by Cardiac Troponin I antibody [N1C3-3] (GTX113028) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

GTX113028 ICC/IF Image

The data was published in the journal PLoS One in 2015. PMID: 25680105

文献引用

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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