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Cardiac Troponin I抗体 [N1C3]

Comparison
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331
Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

目录号 GTX109331

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

预测种属反应

Bovine, Cat, Dog, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Cardiac Troponin I Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952327

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

功能与背景

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500.
Scale bar = M μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cardiac Troponin I antibody [N1C3] used in Western Blot (WB). GTX109331

GTX109331 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109331

GTX109331 IHC-P Image

Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Shan SS et al. IEEE Trans Biomed Circuits Syst 2020; 14 (6) : 1362-1370 A Two-Electrode, Double-Pulsed Sensor Readout Circuit for Cardiac Troponin I Measurement.
Crestani T et al. Biochem Biophys Res Commun 2020; 533 (3) : 376-382 Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells.
实验应用 : WB
种属反应 : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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