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Cas9抗体 [7A9]

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807
Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

目录号 GTX53807

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 7A9

同种型

 IgG1

实验应用

 WB, ICC/IF, IP
产品包装
25 μg (¥2800)
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产品应用

应用说明

Western Blotting Analysis: 0.5 μg/mL from a representative lot detected Cas9 in 1 μg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

产品说明

This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9)

产品属性

形式

Liquid

存储溶液

0.1M Tris-Glycine (pH7.4), 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原

N-Terminal domain of Cas9.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_2887823

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807

GTX53807 IP Image

IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.

Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

GTX53807 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).

文献引用

Application Reference

Zhao C et al. Nat Commun 2020; 11 (1) : 5178 CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication.
Shen CC et al. Nucleic Acids Res 2018; (Epub) Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation.
实验应用 : WB
Germano G et al. Nature 2017; 552 (7683) : 116-120 Inactivation of DNA repair triggers neoantigen generation and impairs tumour growth.
实验应用 : WB
Guo J et al. Protein Cell 2017; (Epub) An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells.
实验应用 : WB
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产品包装 报价 (¥)
¥ 2800
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