Western Blotting Analysis: 0.5 μg/mL from a representative lot detected Cas9 in 1 μg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9)
Buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Batch dependent (Please refer to the vial label for the specific concentration.)
N-Terminal domain of Cas9.
Protein G purified