产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Mouse, Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910078
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
caspase 3 , CPP32 , CPP32B , SCA-1
细胞定位
Cytoplasm
功能与背景
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/templates/default/js/lightbox/images/loading.gif "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
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GTX636810 WB Image
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636810 IHC-P Image
Caspase 3 antibody [HL1370] detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Caspase 3 stained by Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636810 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636810 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 (cleaved Asp175) antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636810 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220513_WB_KO_watermark_w_23061202_600.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220527_IHC-P_22062121_242.webp "Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_T-44599_20220408_WB_R_treatment_Cisplatin_w_23061202_716.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_T-44599_20220318_WB_R_w_23061202_829.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220429_WB_treatment_Cisplatin_w_23061202_958.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220513_WB_KO_watermark_w_23061202_600.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220527_IHC-P_22062121_242.webp "Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_T-44599_20220408_WB_R_treatment_Cisplatin_w_23061202_716.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_T-44599_20220318_WB_R_w_23061202_829.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220429_WB_treatment_Cisplatin_w_23061202_958.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
