产品说明
摘要
Caspase 3 antibody detects caspase 3 protein, a 32 kDa zymogen (also known as pro-caspase 3) that is cleaved at conserved aspartic residues into 17 kDa and 12 kDa subunits upon activation. The cleaved subunits then form an active heterotetramer by hydrophobic interactions and trigger subsequent caspase cascades to induce the apoptotic phenotype.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:10000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Jurkat , Raji , NCI-H929 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 (30uM cisplatin treatment for 24h) , HeLa
产品说明
IP/MS validation was supported by references (PMID:30377401)
预测种属反应
Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10722709
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
caspase 3 , CPP32 , CPP32B , SCA-1
细胞定位
Cytoplasm
功能与背景
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110543 WB Image
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 IHC-P Image
Caspase 3 antibody detects Caspase 3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110543 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 IHC-P Image
Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Caspase 3 antibody (GTX110543) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110543 WB Image
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 12% SDS-PAGE Caspase 3 antibody (GTX110543) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 WB Image
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 WB Image
Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110543 IP Image
Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 μg of Caspase 3 antibody (GTX110543). Western blot analysis was performed using Caspase 3 antibody (GTX110543). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX110543 IHC-P Image
Caspase 3 antibody detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110543 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 28467781
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GTX110543 WB Image
The data was published in the 2020 in Theranostics. PMID: 32550910
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GTX110543 WB Image
The data was published in the 2019 in PLoS Pathog. PMID: 31841561
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GTX110543 WB Image
The data was published in the 2019 in Int J Mol Sci. PMID: 31717306
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GTX110543 WB Image
The data was published in the journal Nutrients in 2019.PMID: 31905929
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GTX110543 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28928421
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GTX110543 WB Image
The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
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GTX110543 WB Image
The data was published in the journal Biomolecules in 2020.PMID: 32635653
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GTX110543 WB Image
The data was published in the journal PLoS One in 2017. PMID: 29045486
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GTX110543 WB Image
The data was published in the journal Toxins (Basel) in 2013. PMID: 24335753
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GTX110543 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26716417
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文献引用
产品评论
Caspase 3 antibody
目录号 GTX110543
Rating
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( Average 4.2 based on 9 users reviews)
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实验应用
Western Blot(WB)
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( Average 4.1 based on 8 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 14-Feb-2023
Application Tested :
WB
Sample Species :
Hu
Sample :
H1299 cell lysates
Blocking :
HyBlock 1min Blocking Buffer, 25°C, 0.01Hr
Primary Antibody :
1:1000, 15°C, 4Hr
Date :
Anonymous submitted on 14-May-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
5 μL
Blocking :
3% BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 28-Dec-2020
Application Tested :
IHC-P
Sample Species :
Rat
Sample :
Injured rat spinal cord tissue
Blocking :
1% BSA in PBS, 25°C, 1Hr
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
Commercial antigen retrieval buffer (pH 7.0)
Primary Antibody :
1:100, 4°C, 24Hr
Figure Note :
Green: Caspase 3, Blue: DAPI
Date :
Anonymous submitted on 27-Dec-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
Luc-MKN45 cells
Amount used :
20 μg
Blocking :
5%FBS in 0.1%PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 22-Jun-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
HEK293 cells
Amount used :
20 μg
Blocking :
5% non-fat milk in TBST, 22°C, 1Hr
Primary Antibody :
1:3000, 4°C, 16Hr
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MG63 cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date :
Anonymous submitted on 04-Jan-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
Mahlavu cells
Amount used :
50μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Date :
Anonymous submitted on 07-Jul-2015
Application Tested :
WB
Sample Species :
Rat
Sample :
C6 cells
Amount used :
30μg
Blocking :
5% milk, 37°C, 1Hr
Primary Antibody :
1:1000, 4°C, 8Hr
Date :
Anonymous submitted on 09-Dec-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
PA-1 cells
Amount used :
50μg
Blocking :
5% milk, 25°C, 0.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr