产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.74 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Caspase 8. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036448
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
细胞定位
Cytoplasm
功能与背景
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110723 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110723 WB Image
Wild-type (WT) and CASP8 (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110723 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110723 ICC/IF Image
Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: Caspase 8 protein stained by Caspase 8 antibody (GTX110723) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX110723 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110723 IHC-P Image
Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110723 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX110723 IHC-P Image
Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110723 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110723 ICC/IF Image
Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:100.Blue: Hoechst 33342 staining.
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GTX110723 WB Image
The data was published in the journal Nutrients in 2018. PMID: 30297634
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GTX110723 WB Image
The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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GTX110723 WB Image
The data was published in the journal Biomolecules in 2020.PMID: 32635653
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GTX110723 WB Image
The data was published in the journal Nutrients in 2018. PMID: 30297634
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文献引用
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