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Chk1抗体 [6F5]

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70303

目录号 GTX70303

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 6F5

同种型

 IgG1

实验应用

 WB, ICC/IF

种属反应

 Human
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

产品说明

Human Chk1

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full-length human Chk1

纯化方式

Protein G affinity purified
From hybridoma culture supernatant

偶联

Unconjugated

RRID

AB_377598

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

checkpoint kinase 1 , CHK1

细胞定位

Nucleus , Cytoplasm

功能与背景

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

数据库

研究领域

產品图片

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Chk1 antibody [6F5] detects Chk1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
10 % SDS-PAGE
Chk1 antibody [6F5] (GTX70303) dilution: 1:500

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 antibody [6F5] (GTX70303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Detection of human Chk1 protein in HeLa whole cell lysate using GeneTex Chk 1 6F5 monoclonal antibody GTX70303

Anti-Chk1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70303

GTX70303 ICC/IF Image

Chk1 antibody [6F5] detects Chk1 protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 stained by Chk1 antibody [6F5] (GTX70303) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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