Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using (GTX70295) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (GTX70295) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal PLoS One in 2014. PMID: 24933654