应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2, MCF-7, or LN-Cap total cell extract
产品说明
GTX70295 is specific for human CHK2 protein.
形式
Liquid
存储溶液
1XPBS (pH7), 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant CHK2 protein
纯化方式
Protein G purified
偶联
Unconjugated
RRID
AB_369315
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1
细胞定位
nucleoplasm
功能与背景
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
数据库
研究领域
![Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295](/upload/website/prouct_img/small/GTX70295/GTX70295_44482_20211112_WB_w_23061221_684.webp "Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295")
![Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295](/upload/website/prouct_img/small/GTX70295/CHK2-antibody-8F12-GTX70295-ICC_IF-1_w_23061221_180.webp "Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295")
![Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295](/upload/website/prouct_img/small/GTX70295/GTX70295_20240411_citeab_WB_2118_24041502_989.webp "Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295")
![Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295](/upload/website/prouct_img/normal/GTX70295/GTX70295_44482_20211112_WB_w_23061221_684.webp "Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295")
![Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295](/upload/website/prouct_img/normal/GTX70295/CHK2-antibody-8F12-GTX70295-ICC_IF-1_w_23061221_180.webp "Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295")
![Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295](/upload/website/prouct_img/normal/GTX70295/GTX70295_20240411_citeab_WB_2118_24041502_989.webp "Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295")
