产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6
细胞定位
Nucleus,Cytosol
功能与背景
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
数据库
研究领域
產品图片
![Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915](/templates/default/js/lightbox/images/loading.gif "Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915")
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GTX637915 ICC/IF Image
Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637915 IHC-P Image
Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:100.Red: Cytoskeleton, a GTX631836 marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836_41855, 1, 500) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637915 IHC-P Image
Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse spinal cord.Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637915 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637915 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915](/upload/website/prouct_img/small/GTX637915/GTX637915_T-44872_20230119_ICC_IF_R_23021401_554.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915](/upload/website/prouct_img/small/GTX637915/GTX637915_T-44872_20221230_IHC-P_M_1_23020621_361.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915](/upload/website/prouct_img/small/GTX637915/GTX637915_T-44872_20221230_IHC-P_M_23020621_393.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915](/upload/website/prouct_img/small/GTX637915/GTX637915_T-44872_20221209_WB_M_tissue_22121123_506.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915](/upload/website/prouct_img/small/GTX637915/GTX637915_44935_20230714_WB_B_23071822_912.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915](/upload/website/prouct_img/normal/GTX637915/GTX637915_T-44872_20230119_ICC_IF_R_23021401_554.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915](/upload/website/prouct_img/normal/GTX637915/GTX637915_T-44872_20221230_IHC-P_M_1_23020621_361.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915](/upload/website/prouct_img/normal/GTX637915/GTX637915_T-44872_20221230_IHC-P_M_23020621_393.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915](/upload/website/prouct_img/normal/GTX637915/GTX637915_T-44872_20221209_WB_M_tissue_22121123_506.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915")
![Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915](/upload/website/prouct_img/normal/GTX637915/GTX637915_44935_20230714_WB_B_23071822_912.webp "Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915")
