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Choline Acetyltransferase抗体 [N1N3]

Overexpression
Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

目录号 GTX113164

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Choline Acetyltransferase 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

83 kDa. ( Note )

预测种属反应

Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949973

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6

功能与背景

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164

GTX113164 IHC-Fr Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164

GTX113164 IHC-Fr Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Red: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:300.
Blue: Hoechst 33342 staining.

Anti-Choline Acetyltransferase antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113164

GTX113164 ICC/IF Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164

GTX113164 IHC-P Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:615.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Ben-Zvi H et al. Int J Mol Sci 2022; 23 (24) PLEKHM2 Loss of Function Impairs the Activity of iPSC-Derived Neurons via Regulation of Autophagic Flux.
Kinga Moln?r et al. J Neuroinflammation 2022; 19 (1) : 68 Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury.
实验应用 : IHC-Fr
种属反应 : Mouse
Bern?t N?gr?di et al. Front Pharmacol 2020; 11 : 584184 Upregulation of Nucleotide-Binding Oligomerization Domain-, LRR- and Pyrin Domain-Containing Protein 3 in Motoneurons Following Peripheral Nerve Injury in Mice.
实验应用 : IHC-Fr
种属反应 : Mouse
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Choline Acetyltransferase antibody [N1N3] 目录号 GTX113164

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实验应用
IHC (Frozen sections)(IHC-Fr)
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Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164
Date : Bernat Nogradi submitted on 22-Mar-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Medulla oblongata region of mouse brain (hypoglossal and vagal nucleus)
Fixation : 4% PFA in PBS, 1 day postfixation
Permeabilization /Antigen retrieval : 0.2% Tritox X-100 in PBS
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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