SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Chromogranin A antibody detects Chromogranin A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Chromogranin A stained by Chromogranin A antibody (GTX113165) diluted at 1:2000 and competitor's antibody (sc-1488) diluted at 1:20.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min