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Chromogranin C抗体

Overexpression
Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446
Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446
Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446
Anti-Chromogranin C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116446

目录号 GTX116446

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Chromogranin C 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

71 kDa. ( Note )

阳性对照

IMR32 , U87-MG , mouse brain , rat brain

预测种属反应

Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Chromogranin C. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11171949

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

secretogranin II , CHGC , EM66 , SN , SgII

细胞定位

Secreted

功能与背景

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446

GTX116446 WB Image

Chromogranin C antibody detects SCG2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
Chromogranin C antibody (GTX116446) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446

GTX116446 WB Image

Chromogranin C antibody detects SCG2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
Chromogranin C antibody (GTX116446) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446

GTX116446 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446

GTX116446 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
GTX116446 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446

GTX116446 IHC-P Image

Chromogranin C antibody detects Chromogranin C protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446

GTX116446 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446

GTX116446 IHC-P Image

The data was published in the journal PLoS One in 2014. PMID: 24558503

Anti-Chromogranin C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116446

GTX116446 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 24558503

文献引用

Application Reference

Lin Z et al. Adv Sci (Weinh) 2022; 9 (27) : e2202263 Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation.
实验应用 : WB
Federico A et al. Mol Ther Oncolytics 2020; 18 : 83-99 Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma.
Xiang H et al. J Int Med Res 2020; 48 (9) : 300060520957541 Characterization of blood-derived exosomal proteins after exercise.
实验应用 : WB
种属反应 : Mouse
Moro A et al. PLoS Biol 2020; 18 (8) : e3000826 CaMKII controls neuromodulation via neuropeptide gene expression and axonal targeting of neuropeptide vesicles.
实验应用 : WB
种属反应 : Mouse
Valiya Veettil M et al. J Virol 2020; (Epub) Kaposi's Sarcoma Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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