产品说明
摘要
Citrate Antigen Retrieval Buffer, 10X pH6.0
产品应用
应用说明
Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. The tissue sections are ready for further IHC protocol.
产品属性
形式
Liquid
存放说明
Store at 2-8ºC, DO NOT FREEZE.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
文献引用
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产品评论
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