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Citrate synthase抗体 [GT1761]

KO/KD Orthogonal
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

目录号 GTX628143

文献引用 6

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT1761

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

Citrate synthase antibody detects citrate synthase, the 52 kDa enzyme that catalyzes the first reaction in the citric acid cycle (Krebs cycle) to convert acetyl-CoA and oxaloacetate to citrate and CoA-SH. Citrate synthase exists within the mitochondrial matrix in eukaryotic cells and is used commonly as a marker of intact mitochondria.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

zebrafish brain , 293T , A431 , HeLa , HepG2 , A375 , mouse brain , zebrafish heart , rat brain

预测种属反应

Bovine, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888020

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

citrate synthase

细胞定位

Mitochondrion matrix

功能与背景

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg zebrafish brain lysate/extract
10% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Citrate synthetase antibody (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg zebrafish heart lysate/extract
10% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628143

GTX628143 ICC/IF Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Pablo Ranea-Robles et al. Cell Mol Life Sci 2021; 78 (14) : 5631-5646 Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.
实验应用 : WB
种属反应 : Mouse
Pablo Ranea-Robles et al. Kidney360 2021; 2 (9) : 1441-1454 Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.
实验应用 : WB
种属反应 : Mouse
Leandro J et al. Hum Mol Genet 2020; 29 (7) : 1168-1179 DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.
Zhang X et al. J Biol Chem 2020; 295 (23) : 8078-8095 Structure-function analyses of the G729R 2-oxoadipate dehydrogenase genetic variant associated with a disorder of l-lysine metabolism.
实验应用 : WB
Jo?o Leandro et al. bioRxiv 2019; (Epub) DHTKD1 and OGDH display in vivo substrate overlap and form a hybrid ketoacid dehydrogenase complex.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
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¥ 1700
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