产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
zebrafish heart , 293T , A431 , HepG2 , mouse brain , zebrafish brain , rat brain
预测种属反应
Bovine, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888021
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
citrate synthase
细胞定位
Mitochondrion matrix
功能与背景
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/templates/default/js/lightbox/images/loading.gif "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
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GTX628144 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT2061] (GTX628144) diluted at 1:1000.
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GTX628144 WB Image
Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 30 μg zebrafish heart lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000
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GTX628144 WB Image
Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 30 μg zebrafish brain lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000
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GTX628144 IHC-P Image
Citrate synthetase antibody [GT2061] detects CS protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628144 ICC/IF Image
Citrate synthetase antibody [GT2061] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT2061] (GTX628144) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX628144 WB Image
Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000
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|
|
GTX628144 WB Image
Citrate synthetase antibody detects CS protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody (GTX628144) dilution: 1:1000
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文献引用
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![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41148_20160114_WB_R_brain_w_23061202_436.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41148_WB_Z_heart_w_23061202_476.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41148_WB_Z_brain_w_23061202_303.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41183_IHC_w_23061202_589.webp "Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41183_20170913_IFA_w_23061202_356.webp "Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41148_WB_M_brain_w_23061202_534.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/small/GTX628144/GTX628144_41148_WB_w_23061202_261.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41148_20160114_WB_R_brain_w_23061202_436.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41148_WB_Z_heart_w_23061202_476.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41148_WB_Z_brain_w_23061202_303.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41183_IHC_w_23061202_589.webp "Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41183_20170913_IFA_w_23061202_356.webp "Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41148_WB_M_brain_w_23061202_534.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
![Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144](/upload/website/prouct_img/normal/GTX628144/GTX628144_41148_WB_w_23061202_261.webp "Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144")
