GeneTex中国官方网站
  • China

Citrate synthase抗体 [N2C3]

KO/KD
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

目录号 GTX110624

文献引用 24

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP

种属反应

 Human, Mouse, Rat, Zebrafish, Chicken, Monkey, Frog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Citrate synthase 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

mouse brain , rat brain , Rat muscle , 293T

预测种属反应

Bovine, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950045

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

citrate synthase

细胞定位

Mitochondrion matrix

功能与背景

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624

GTX110624 IP Image

Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624).
Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Citrate synthetase antibody detects Citrate synthetase protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) at a dilution of 1:1000.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Citrate synthetase antibody [N2C3] (GTX110624) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cs antibody (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624

GTX110624 ICC/IF Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624

GTX110624 IHC-Wm Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624

GTX110624 IHC-Wm Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25489948

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28515438

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28515438

文献引用

Application Reference

Todorova V et al. Mol Neurodegener 2023; 18 (1) : 15 Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation.
实验应用 : IHC-Fr
种属反应 : Mouse
Lin CC et al. EMBO Rep 2022; 23 (12) : e55191 Parkin coordinates mitochondrial lipid remodeling to execute mitophagy.
Demchenko IT et al. Front Mol Neurosci 2022; 15 : 1062410 GAT inhibition preserves cerebral blood flow and reduces oxidant damage to mitochondria in rodents exposed to extreme hyperbaric oxygen.
Todorova V et al. Sci Rep 2022; 12 (1) : 20195 Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.
Katharina I Zittlau et al. Mol Cell Proteomics 2022; 21 (2) : 100191 Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
实验应用 : WB, ICC/IF
种属反应 : Human
显示更多 +
我要回报文献

产品评论

目前没有使用 Citrate synthase antibody [N2C3] (GTX110624) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
Application Reference
Todorova V et al. Mol Neurodegener 2023; 18 (1):15 Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation.
实验应用 : IHC-Fr
种属反应 : Mouse
Lin CC et al. EMBO Rep 2022; 23 (12):e55191 Parkin coordinates mitochondrial lipid remodeling to execute mitophagy.
Demchenko IT et al. Front Mol Neurosci 2022; 15:1062410 GAT inhibition preserves cerebral blood flow and reduces oxidant damage to mitochondria in rodents exposed to extreme hyperbaric oxygen.
Todorova V et al. Sci Rep 2022; 12 (1):20195 Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.
Katharina I Zittlau et al. Mol Cell Proteomics 2022; 21 (2):100191 Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
实验应用 : WB, ICC/IF
种属反应 : Human
Hagir Suliman et al. Front Physiol 2021; 12:683098 Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney Injury.
实验应用 : IHC-P
种属反应 : Mouse
Leandro J et al. Hum Mol Genet 2020; 29 (7):1168-1179 DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.
Ding CC et al. Nat Metab 2020; 2 (3):270-277 MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis.
Al-Attar R et al. Gene 2020; 761:145039 RAGE against the stress: Mitochondrial suppression in hypometabolic hearts.
实验应用 : WB
种属反应 : Frog
Chao-Chieh Lin et al. bioRxiv 2020; (Epub) Adipose-Derived Stem Cells Modulate BV2 Microglial M1/M2 Polarization by Producing Glial Cell-Derived Neurotrophic Factor.
实验应用 : WB
种属反应 : Human
Jo?o Leandro et al. bioRxiv 2019; (Epub) DHTKD1 and OGDH display in vivo substrate overlap and form a hybrid ketoacid dehydrogenase complex.
实验应用 : WB
种属反应 : Human
Leandro J et al. Mol Genet Metab 2019; (Epub) Mild inborn errors of metabolism in commonly used inbred mouse strains.
实验应用 : WB
种属反应 : Mouse
Ding C.C. et al. bioRxiv 2018; (Epub) Mammalian stringent-like response mediated by the cytosolic NADPH phosphatase MESH1.
实验应用 : WB
种属反应 : Human
Nam M et al. Sci Rep 2017; 7 (1):2013 Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression.
Suliman HB et al. JCI Insight 2017; 2 (3):e89676 Mitochondrial quality-control dysregulation in conditional HO-1-/- mice.
Kim Y et al. Appl Physiol Nutr Metab 2017; (Epub) Effects of long-term exposures to low iron and branched-chain amino acid containing diets on aging skeletal muscle of Fisher 344 Brown Norway rats.
实验应用 : WB
种属反应 : Rat
Lake NJ et al. Am J Hum Genet 2017; 101 (2):239-254 Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.
实验应用 : WB
种属反应 : Human
Baati N et al. Biochim Biophys Acta 2017; Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles.
实验应用 : WB
种属反应 : Mouse
Sanchez I et al. Hum Mol Genet 2016; Ataxin-1 regulates the cerebellar bioenergetics proteome through the GSK3β-mTOR pathway which is altered in Spinocerebellar ataxia type 1 (SCA1).
实验应用 : WB
种属反应 : Mouse
Fredenburgh LE et al. Am J Physiol Lung Cell Mol Physiol 2015; 309 (8):L834-46 Effects of inhaled CO administration on acute lung injury in baboons with pneumococcal pneumonia.
实验应用 : WB
种属反应 : Monkey
Abdel Khalek W et al. PLoS One 2014; 9 (12):e114388 SIRT3, a Mitochondrial NAD+-Dependent Deacetylase, Is Involved in the Regulation of Myoblast Differentiation.
实验应用 : WB
种属反应 : Mouse
Alan L. Chang et al. Free Radical Biology and Medicine 2014; Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis
实验应用 : ICC/IF
种属反应 : Mouse
Mizushima S et al. Development 2014; 141 (19):3799-806 The birth of quail chicks after intracytoplasmic sperm injection.
实验应用 : WB
种属反应 : Chicken
Rosenkilde M et al. Scand J Med Sci Sports 2013; Changes in peak fat oxidation in response to different doses of endurance training.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com