产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
预测种属反应
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888056
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cofilin 1 , CFL , HEL-S-15 , cofilin
细胞定位
Nucleus matrix , Cytoplasm , cytoskeleton
功能与背景
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]
数据库
研究领域
產品图片
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/templates/default/js/lightbox/images/loading.gif "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
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GTX628804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX628804 IHC-P Image
Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody [GT567] (GTX628804) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 ICC/IF Image
Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_w_23061202_779.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_IHC_w_23061202_146.webp "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_R_w_23061202_195.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_M_w_23061202_861.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20150807_IFA_w_23061202_961.webp "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_w_23061202_779.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_IHC_w_23061202_146.webp "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_R_w_23061202_195.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_M_w_23061202_861.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20150807_IFA_w_23061202_961.webp "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
