产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human COL3A1 protein, and it does not cross react with human COL2A1, COL1A2, and COL1A1 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Collagen III
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
collagen type III alpha 1 chain , EDS4A , EDSVASC
细胞定位
Secreted, extracellular space, extracellular matrix
功能与背景
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
数据库
研究领域
產品图片
![Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655](/templates/default/js/lightbox/images/loading.gif "Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655")
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GTX637655 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637655 IHC-P Image
Collagen III antibody [HL1906] detects Collagen III protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.Collagen III stained by Collagen III antibody [HL1906] (GTX637655) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637655 ICC/IF Image
Collagen III antibody [HL1906] detects Collagen III protein at cytoplasmic vesicle by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.Green: Collagen III stained by Collagen III antibody [HL1906] (GTX637655) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637655 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
产品评论
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![Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655](/upload/website/prouct_img/small/GTX637655/GTX637655_44963_20230331_WB_multiple_B_23041023_102.webp "Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655")
![Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655](/upload/website/prouct_img/small/GTX637655/GTX637655_T-44837_20221202_IHC-P_M_22122722_350.webp "Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655")
![Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655](/upload/website/prouct_img/small/GTX637655/GTX637655_44963_20230512_ICC_IF_23060622_581.webp "Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655")
![Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655](/upload/website/prouct_img/small/GTX637655/GTX637655_44963_20230224_WB_TPM_watermark_23030219_193.webp "Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655")
![Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655](/upload/website/prouct_img/normal/GTX637655/GTX637655_44963_20230331_WB_multiple_B_23041023_102.webp "Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655")
![Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655](/upload/website/prouct_img/normal/GTX637655/GTX637655_T-44837_20221202_IHC-P_M_22122722_350.webp "Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655")
![Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655](/upload/website/prouct_img/normal/GTX637655/GTX637655_44963_20230512_ICC_IF_23060622_581.webp "Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655")
![Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655](/upload/website/prouct_img/normal/GTX637655/GTX637655_44963_20230224_WB_TPM_watermark_23030219_193.webp "Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655")
