产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human Connexin 43. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886808
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
gap junction protein alpha 1 , AVSD3 , CMDR , CX43 , EKVP , EKVP3 , GJAL , HLHS1 , HSS , ODDD , PPKCA
细胞定位
Cell membrane,Endoplasmic reticulum
功能与背景
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]
数据库
研究领域
產品图片
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GTX133022 IHC-P Image
Connexin 43 antibody detects Connexin 43 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133022 ICC/IF Image
Connexin 43 antibody detects Connexin 43 protein by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:500.Red: phalloidin, a cytoskeleton marker diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX133022 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133022 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133022 ICC/IF Image
Connexin 43 antibody detects Connexin 43 protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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