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Cre recombinase抗体 [GT10212]

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

目录号 GTX629479

文献引用 2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT10212

同种型

 IgG2a

实验应用

 WB, IP
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:20000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

39 kDa. ( Note )

阳性对照

Cre-tagged protein expressing HeLa cell

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888117

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

数据库

研究领域

產品图片

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479

GTX629479 IP Image

Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody (GTX629479) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX629479) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells
7.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Wang Z et al. Vaccines (Basel) 2021; 9 (7) In Vivo Production of HN Protein Increases the Protection Rates of a Minicircle DNA Vaccine against Genotype VII Newcastle Disease Virus.
Jiang Y et al. Appl Environ Microbiol 2019; (Epub) A novel Cre recombinase-mediated in vivo minicircle DNA (CRIM) vaccine provides partial protection against Newcastle disease virus.
实验应用 : WB
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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