产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa
预测种属反应
Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.71 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10731275
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
creatine kinase, M-type , CKMM , CPK-M , M-CK
细胞定位
Cytoplasm
功能与背景
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX103792 IHC-P Image
Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat muscle.Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103792 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103792 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103792 WB Image
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX103792 diluted at 1:1000
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文献引用
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产品评论
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