Creatine kinase (muscle) antibody [N2C3] detects Creatine kinase (muscle) protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
GTX108969 diluted at 1:1000

Creatine kinase (muscle) antibody detects Creatine kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted by 1:1000.

Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX108969) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:300 dilution.

Creatine kinase (muscle) antibody [N2C3] detects Ckma protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:100.

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:200 dilution.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.