Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 μg of Cyclin D1 antibody [N1C3] (GTX108624).
Western blot analysis was performed using Cyclin D1 antibody [N1C3] (GTX108624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Cyclin D1 antibody [N1C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Sandwich ELISA detection of recombinant full-length Cyclin D1 protein using GTX634347 as capture antibody at concentration of 5 μg/mL and GTX108624 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Cyclin D1 stained by Cyclin D1 antibody (GTX108624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701