产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , Molt-4 , Raji , 293T
预测种属反应
Rabbit, Sheep, Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Cyclophilin A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036709
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
peptidylprolyl isomerase A , CYPA , CYPH , HEL-S-69p
细胞定位
Cytoplasm
功能与背景
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113520 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [N1C3] (GTX113520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113520 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [N1C3] (GTX113520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113520 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cyclophilin A(GTX113520) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113520 WB Image
Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX113520) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113520 WB Image
Sample (50 μg of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113520 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113520 WB Image
Sample (30 μg of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX113520 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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