产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human Cytochrome C recombinant protein
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cytochrome c, somatic , CYC , HCS , THC4
功能与背景
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
数据库
研究领域
產品图片
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/templates/default/js/lightbox/images/loading.gif "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
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GTX638209 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638209 WB Image
Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638209 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638209 IHC-P Image
Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638209 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638209 IHC-P Image
Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638209 ICC/IF Image
Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638209 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_45012_20230707_WB_shRNA_watermark_23071300_650.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_45012_20230519_WB_R_23053001_233.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_45012_20230519_WB_R_brain_23053001_262.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_45012_20230721_IHC-P_Cat_23073119_569.webp "Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_T-44942_20230210_WB_M_brain_23021401_315.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_T-44942_20230303_IHC-P_23031402_575.webp "Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_T-44942_20230331_ICC_IF_23041023_864.webp "Anti-Cytochrome C antibody [HL2205] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/small/GTX638209/GTX638209_45012_20230421_WB_TPM_watermark_23042500_470.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230707_WB_shRNA_watermark_23071300_650.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230519_WB_R_23053001_233.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230519_WB_R_brain_23053001_262.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230721_IHC-P_Cat_23073119_569.webp "Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_T-44942_20230210_WB_M_brain_23021401_315.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_T-44942_20230303_IHC-P_23031402_575.webp "Anti-Cytochrome C antibody [HL2205] used in IHC (Paraffin sections) (IHC-P). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_T-44942_20230331_ICC_IF_23041023_864.webp "Anti-Cytochrome C antibody [HL2205] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638209")
![Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209](/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230421_WB_TPM_watermark_23042500_470.webp "Anti-Cytochrome C antibody [HL2205] used in Western Blot (WB). GTX638209")
