Cytochrome C抗体

Name: Cytochrome C抗体
Brand: GeneTex
SKU: GTX108585
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

Anti-Cytochrome C antibody used in IHC (Paraffin sections) (IHC-P). GTX108585

目录号 GTX108585

文献引用 33

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr
种属反应	Human, Mouse, Rat, Drosophila, Dog
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Cytochrome C 的产品

View product citations for antibody GTX108585 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

12 kDa. ( Note )

阳性对照

Mouse brain , PC-12 , K562 , THP-1 , HL-60 , 293T

预测种属反应

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Cytochrome C Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036712

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

cytochrome c, somatic , CYC , HCS , THC4

细胞定位

Mitochondrion matrix

功能与背景

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

数据库

Gene ID: 54205 CYCS
UniProt: P99999 CYCS

研究领域

產品图片

GTX108585 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 WB Image

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.

GTX108585 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108585 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX108585 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28928421

文献引用

Application Reference

Ogasawara A et al. Fujita Med J 2023; 9 (2) : 147-153 Agaritine derived from Agaricus blazei Murrill induces apoptosis via mitochondrial membrane depolarization in hematological tumor cell lines.

Garc?a-Arroyo R et al. Int J Mol Sci 2022; 23 (19) CERKL, a Retinal Dystrophy Gene, Regulates Mitochondrial Transport and Dynamics in Hippocampal Neurons.

实验应用 : WB

种属反应 : Mouse

Wang CH et al. J Am Heart Assoc 2022; 11 (21) : e027685 Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia-Induced Cardiac Arrest and Resuscitation Rat Model.

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Huang FL et al. Oncol Rep 2022; 47 (2) Niclosamide suppresses T?cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy.

实验应用 : WB

种属反应 : Human

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产品评论

Cytochrome C antibody 目录号 GTX108585

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 10-Jan-2014

Score :

Application Tested : WB

Sample Species : Hu

Sample : SCC-25 cells

Amount used : 25μg

Blocking : 5% non-fat milk, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 07-May-2012

Score :

Application Tested : WB

Sample Species : Hu

Sample : 293T cells

Amount used : 40μg

Blocking : 5% milk in TBST, 25°C, 1Hr

Primary Antibody : 1:1000, 25°C, 16Hr

我要评论产品

Application Reference

Ogasawara A et al. Fujita Med J 2023; 9 (2):147-153 Agaritine derived from Agaricus blazei Murrill induces apoptosis via mitochondrial membrane depolarization in hematological tumor cell lines.

Garc?a-Arroyo R et al. Int J Mol Sci 2022; 23 (19) CERKL, a Retinal Dystrophy Gene, Regulates Mitochondrial Transport and Dynamics in Hippocampal Neurons.

实验应用 : WB

种属反应 : Mouse

Wang CH et al. J Am Heart Assoc 2022; 11 (21):e027685 Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia-Induced Cardiac Arrest and Resuscitation Rat Model.

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Huang FL et al. Oncol Rep 2022; 47 (2) Niclosamide suppresses T?cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy.

实验应用 : WB

种属反应 : Human

Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Erin Clough et al. J Neuroimmune Pharmacol 2021; 16 (4):770-784 Mitochondrial Dynamics in SARS-COV2 Spike Protein Treated Human Microglia: Implications for Neuro-COVID.

实验应用 : ICC/IF

种属反应 : Human

Hongzhi Hu et al. J Nanobiotechnology 2021; 19 (1):390 Mitochondria-targeted accumulation of oxygen-irrelevant free radicals for enhanced synergistic low-temperature photothermal and thermodynamic therapy.

实验应用 : WB

种属反应 : Human

Yang DQ et al. Oxid Med Cell Longev 2021; 2021:6644238 Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.

实验应用 : WB

Liao MF et al. Biomedicines 2021; 9 (5) Interactions between Autophagy, Proinflammatory Cytokines, and Apoptosis in Neuropathic Pain: Granulocyte Colony Stimulating Factor as a Multipotent Therapy in Rats with Chronic Constriction Injury.

实验应用 : WB

种属反应 : Rat

Kunzke T et al. J Cachexia Sarcopenia Muscle 2020; 11 (1):226-240 Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.

Cornelissen T et al. Hum Mol Genet 2020; (Epub) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2.

实验应用 : WB

种属反应 : Human

Thomas Kunzke et al. Journal of Cachexia, Sarcopenia and Muscle 2020; (Epub) Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.

实验应用 : IHC-P

种属反应 : Mouse

Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281

Romero MA et al. Am J Physiol Cell Physiol 2019; (Epub) Five months of voluntary wheel running downregulates skeletal muscle LINE-1 gene expression in rats.

实验应用 : WB

种属反应 : Rat

Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.

实验应用 : WB

种属反应 : Human

Huang SH et al. Phytomedicine 2019; 64:153068 Rooibos suppresses proliferation of castration-resistant prostate cancer cells via inhibition of Akt signaling.

实验应用 : WB

种属反应 : Human

Kuriyama S et al. Cell Death Discov 2018; 4:11 PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.

Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.

实验应用 : WB

种属反应 : Human

Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.

实验应用 : WB

种属反应 : Human

Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.

实验应用 : WB

种属反应 : Human

Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.

实验应用 : WB

种属反应 : Mouse

Sharikova AV et al. J Neuroimmune Pharmacol 2018; (Epub)

实验应用 : WB

种属反应 : Human

Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.

实验应用 : WB

种属反应 : Human

Kuriyama S et al. Cell Death Discovery 2018; (Epub) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.

实验应用 : WB

种属反应 : Human

Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.

Li SM et al. Sci Rep 2017; 7 (1):11893 Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.

实验应用 : WB

种属反应 : Human

Tsui KH et al. Reproduction 2017; 154 (2):101-110 DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.

实验应用 : WB

种属反应 : Human

Nehra S et al. High Alt Med Biol 2016; Chronic Hypobaric Hypoxia Induces Right Ventricular Hypertrophy and Apoptosis in Rats: Therapeutic Potential of Nanocurcumin in Improving Adaptation.

实验应用 : IHC-Fr

种属反应 : Rat

Chiang MC et al. Exp Cell Res 2016; 347 (2):322-31 * activation of ampk-dependent pathways is neuroprotective in human neural stem cells against amyloid-beta-induced mitochondrial dysfunction.

实验应用 : WB

种属反应 : Human

Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.

实验应用 : WB

种属反应 : Human

Ruan Y et al. Mol Med Rep 2015; 12 (4):5796-806 Autophagy inhibition enhances isorhamnetin-induced mitochondria-dependent apoptosis in non-small cell lung cancer cells.

Li CJ et al. Sci Rep 2015; 24 (5):9819 Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Cytochrome C抗体

目录号 GTX108585

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX108585 WB Image

GTX108585 WB Image

GTX108585 WB Image

GTX108585 WB Image

GTX108585 IHC-P Image

GTX108585 IHC-P Image

GTX108585 WB Image

GTX108585 WB Image

GTX108585 WB Image

GTX108585 WB Image

文献引用

Application Reference

产品评论

Cytochrome C antibody 目录号 GTX108585

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